Figure 4.
Efficacy of G47Δ in Peritoneal Dissemination Models
(A–C) Female athymic mice were intraperitoneally injected with MKN45-luc cells (5.0 × 106 cells). Mice were divided into two groups on day 3 and then intraperitoneally injected with G47Δ (1 × 106 PFU) or mock on days 4 and 7. (A) The photon counts of peritoneal tumors were calculated with IVIS imaging every 3–4 days. G47Δ treatment significantly decreased the total bioluminescence from disseminated tumors (p < 0.01 on day 24). (B) G47Δ treatment prolonged the survival of tumor-bearing mice (p < 0.01). (C) Two representative cases from each group are shown. (D–F) A peritoneal dissemination model was generated with 44As3Luc cells (1.0 × 106 cells) in female athymic mice, and the same experiment was performed as above. (D) G47Δ treatment significantly decreased the total bioluminescence from disseminated tumors (p < 0.01 on day 7). (E) G47Δ treatment prolonged the survival (p < 0.01) of tumor-bearing mice. (F) Two representative cases from each group are shown. The results presented are the mean ± SEM (n = 11 and n = 10, respectively). A Student’s t test was used to determine the statistical significance (∗∗p < 0.01). Survival was analyzed by the Kaplan-Meier method using the log rank test (∗∗p < 0.01). (G–I) Female athymic mice were intraperitoneally injected with 44As3Luc cells (1.0 × 106 cells). (G) Mice were divided into two groups on day 3 and then intraperitoneally injected with G47Δ (5 × 106 PFU) or mock on days 3, 5, and 7. (H) In the G47Δ treatment group, two mice were cured, two died, and six survived at day 50. All mice died within 30 days in the mock treatment group. (I) G47Δ treatment prolonged the survival of tumor-bearing mice significantly (p < 0.001). (J–L) Female athymic mice were intraperitoneally injected with 44As3Luc cells (1.0 × 106 cells). (J) Mice were intraperitoneally injected with G47Δ (1 × 106 PFU) or mock on days 1, 3, and 5. (K) In the G47Δ treatment group, seven mice were cured, none died, and three survived at day 50. All mice died within 30 days in the mock treatment group. (L) G47Δ treatment prolonged the survival of tumor-bearing mice significantly (p < 0.001).