Figure 7.
Gene Expression Changes in MKN45 Subcutaneous Tumors Treated with G47Δ
Athymic mice bearing MKN45 subcutaneous tumors were inoculated intratumorally with G47Δ (1 × 106 PFU) or mock. Tumor tissues were harvested 2 days after the treatments, total RNA was extracted, cDNA was reverse transcribed, and gene expression analysis was performed. (A–E) Gene expressions of (A) Cd68, (B) Cd86, (C) Cd163, (D) Arg1, and (E) Mrc1 were compared between the two treatment groups. Expression data were normalized to the geometric mean of three housekeeping genes (Actb, Gapdh, and Hprt1). Bar represents mean relative quantification + SEM (n = 6). A Student’s t test was used to determine the statistical significance (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; NS, not significant).