Figure 5.

Anti-Tumor Activity of B7-H3 CAR-T Cells In Vitro

(A) Representative flow cytometry plots of mock or CAR-T cells cocultured with tumor cell lines at an E:T ratio of 4:1 for 24 h. A375, Raji, and MV4-11 cells were identified according to their B7-H3 expression. (B) Secretion of perforin from T cells was detected in the same condition as in (A). (C–F) After coculturing mock or CAR-T cells with tumor cells at an E:T ratio of 4:1 for 12 h, the percentages of residual tumor cells were estimated from the FACS data (C), and the concentrations of IFN-γ (D), IL-2 (E), and TNF-α (F) in supernatants were measured by ELISA kits (D–F). All error bars represent SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, one-way ANOVA with Holm–Sidak adjusted p values.