Table 1. Parameters of the Phosphate Release from the Synthesized Calcium Pyrophosphate by PPase Samples^a.

	samples	[Pi]max [μM]	k [h–1]
control		20 ± 2	0.02 ± 0.01
Ec-PPase	soluble	76 ± 2	0.04 ± 0.01
	immobilized on ND–NH2	62 ± 2	0.04 ± 0.01
	immobilized on ND–NH–(CH2)6–NH2	66 ± 2	0.03 ± 0.01
Mt-PPase	soluble	58 ± 2	0.08 ± 0.01
	immobilized on ND–NH2	62 ± 2	0.09 ± 0.02
	immobilized on ND–NH–(CH2)6–NH2	74 ± 2	0.04 ± 0.01

^aThe conditions included the following: 50 mM Tris-HCl, pH of 7.5, 10 mg mL^–1 Ca₂P₂O₇·2H₂O, 10 mM MgCl₂, 2 mM CaCl₂, and 0.01 μg mL^–1 PPase.