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. 2020 Apr 7;5(15):8564–8571. doi: 10.1021/acsomega.9b04412

Figure 1.

Figure 1

MRM chromatograms for the trypsin-digested murine IgG. (a) MRM chromatograms for the subclass-specific peptides and N-glycopeptides from the trypsin-digested mouse IgG standard. (b) Enlarged detail of the glycopeptide part in (a). (c) MRM chromatograms for the subclass-specific peptides and N-glycopeptides from the trypsin-digested IgG from a C57BL/6 mouse serum sample. (d) Enlarged detail of the glycopeptide part in (c). (e) MRM chromatograms for the subclass-specific peptides and N-glycopeptides from the trypsin-digested IgG from the mixed mouse serum sample. (f) Enlarged detail of the glycopeptide part in (e). Structural symbols: blue square: N-acetylglucosamine; green circle: mannose; red triangle: fucose; yellow circle: galactose; and white diamond: N-glycolylneuraminic acid.