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. 2020 Apr 7;5(15):8564–8571. doi: 10.1021/acsomega.9b04412

Table 1. MRM Transitions for Murine IgG Subclass Monitoring.

IgG subclass peptide precursor ion [M + 2H]2+ (m/z) product ion [M + H]+ (m/z)
IgG1/1* VNSAAFPAPIEK 622.3 654.4
IgG2aA DLPAPIER 455.8 514.3
IgG2c ALPSPIEKa 427.8 335.7 ([M + 2H]2+)
IgG2b/2b* DLPSPIER 463.8 698.4
IgG3 ALPAPIER 433.8 682.4
a

ALPSPIEK exists in both IgG2c and IgG2aB.23