Fig. 8.
Inhibition of recombinant N-type CaV channels by gabapentinoids. (A) Mean IBa density obtained from HEK-293 cells expressing CaV1.3α1/CaVβ3-1 channels in the control condition and after chronic treatment (48 h) with 1 mM GBP or AdGABA using solutions A and D (Table 1). Currents were elicited by a 140 ms depolarizing pulse to +10 mV from a Vh of −80 mV. n = 7–17 recorded cells. (B) Comparison of the time constants of inactivation (τinact) in absence after the exposure to GBP and AdGABA as indicated. The values of τinact were obtained by fitting the decaying phase of current traces with eqn 4. The asterisk denotes significant differences (p < 0.05) compared with control.