Figure 4.

Histatin-5 Accelerates Cell Migration in Standardized Scratch Assays in Multiple Cell Types

Hst5 application led to statistically significant improvement in scratch closure rates in all tested cell types compared to Un controls. (A–C) Cell types and conditions used included (A) HCE cells, (B) HeLa cells, and (C) MCF-7 cells in reduced serum conditions. All experiments were performed three separate times with three technical replicates for each experiment. Statistical significance was determined using the Student’s t test. ∗p < 0.05, ∗∗p < 0.01. Relative closure = (% closure of treated sample)/(% closure of Un control).