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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Mol Microbiol. 2019 Dec 8;113(2):418–429. doi: 10.1111/mmi.14428

Figure 5. Specific interactions between FlcA and other flagellar proteins by affinity blotting.

Figure 5.

Approximately 1 μg of MBP-tagged proteins shown on top of each panel were subjected to SDS-PAGE and then stained with Coomassie blue or transferred to a PVDF membrane. The membranes were incubated with 1xFLAG tagged MotB, FlbB, BB0236 or FliL, and then immunoblotted with anti-FLAG monoclonal antibodies.