Fig. 5.

Infected Tmprss2^−/− mice exhibit an adaptive immune response. ELISA of sera from female 8- to 12-week-old PR8_HA(H2) infected wild type (WT) and Tmprss2^−/− knock-out (KO) mice for anti-IAV antibodies. Mice were infected with 2 × 10⁴ focus forming units (ffu) PR8_HA(H2). At 14 days post infection (dpi), sera from infected and PBS mock-treated mice were prepared and analyzed for the presence of IAV-specific antibodies. Boxplots of OD values at 450 nm and median values per group are shown. WT-PBS: PBS treated control WT mice (n = 7), WT-H2: WT mice infected with 2 × 10⁴ ffu PR8_HA(H2) virus (n = 7), KO-PBS: PBS treated control mice (n = 5), KO-H2: KO mice infected with 2 × 10⁴ ffu with PR8_HA(H2) virus (n = 4). Differences between the means of treated control and infected mice were significant (p < 0.001)