Table 2.
Antimicrobial activity of CHI and CHI/POZ blend films.
| Samples | Chemical Composition (% v/v) | Diameter of Growth Inhibition Zone (mm) | |
|---|---|---|---|
| Staphylococcus aureus | Escherichia coli | ||
| Drug-Free Films | |||
| A | CHI (100) | 0 | 0 |
| B | CHI/POZ (80:20) | 0 | 17.3 ± 2.1*** |
| C | CHI/POZ (60:40) | 0 | 13.3 ± 2.1** |
| D | CHI/POZ (40:60) | 0 | 21.0 ± 2.0**** |
| Drug-Loaded Films (CF 0.1% w/v) | |||
| A1 | CHI (100) | 38.6 ± 1.3*** | 39.5 ± 1.9* |
| B1 | CHI/POZ (80:20) | 36.0 ± 1.9** | 40.1 ± 2.6** |
| C1 | CHI/POZ (60:40) | 38.6 ± 1.6*** | 42.5 ± 2.2*** |
| D1 | CHI/POZ (40:60) | 46.1 ± 1.7*** | 39.3 ± 2.3* |
| E | Disc with CF | 31.2 ± 0.4 | 35.1 ± 1.2 |
CHI, chitosan; POZ, poly(2-ethyl-2-oxazoline); CF, ciprofloxacin. Anti-microbial activity values of CHI/POZ drug-free films and ciprofloxacin-loaded films were compared to pure CHI and discs with CF, respectively. Statistically significant differences are given as: ****—p < 0.0001; ***—p < 0.001; **—p < 0.01; *—p < 0.05. Data are expressed as mean ± standard deviation (n = 3).