Fig. 4.

NEAT1 acted as a miR-599 sponge. a Schematic of the target sequence for miR-599 in NEAT1 and mutated miR-599-binding sequence. b The luciferase activity in SW1736 and KAT-18 cells cotransfected with WT-NEAT1′ or MUT-NEAT1′ and miR-NC mimic or miR-599 mimic. c NEAT1 enrichment in the RISC of SW1736 and KAT-18 cells transfected with miR-NC mimic or miR-599 mimic using anti-Ago2 or IgG antibody. The expression of miR-599 by qRT-PCR in SW1736 and KAT-18 cells transfected with si-NC or si-NEAT1 (d), in 25 pairs ATC tissues and adjacent normal tissues (e), Nthy-ori3-1, SW1736 and KAT-18 cells (f), the two ATC cells exposed to 1% O₂ for various time points (0, 3, 6, 12, 24 and 48 h) (g). h Spearman test for the correlation between NEAT1 level and miR-599 expression in ATC tissues. *P < 0.05