Table 2.
Mean values for HPLC quantification of Chlorogenic acid, Caffeic acid, Kaempferol, Thymoquinone and Disogenin (μg/g) in different spices
| Treatments | Chlorogenic Acid | Caffeic Acid | Kaempferol | Thymoquinone | Disogenin |
|---|---|---|---|---|---|
| T1 | 139.6 ± 7.72a | 83.73 ± 2.39a | 228.6 ± 9.72a | N.D | N.D |
| T2 | 101.5 ± 5.43c | 4.09 ± 0.09e | 6.02 ± 0.03e | 5.13 ± 0.06a | N.D |
| T3 | 40.3 ± 0.79 g | 3.06 ± 0.06 g | 5.59 ± 0.02 g | N.D | 27.72 ± 0.52a |
| T4 | 109.8 ± 6.52b | 22.83 ± 2.37c | 114.6 ± 4.84b | 2.49 ± 0.03c | N.D |
| T5 | 88.6 ± 2.26e | 23.22 ± 2.53b | 112.1 ± 4.62c | N.D | 11.27 ± 0.35c |
| T6 | 68.8 ± 1.59f | 3.46 ± 1.45f | 5.82 ± 0.06f | 2.54 ± 0.02b | 12.31 ± 0.37b |
| T7 | 93.4 ± 2.96d | 30.05 ± 1.36d | 81.5 ± 1.39d | 1.69 ± 0.01d | 8.39 ± 0.15d |
One way anova was applied to check the overall behavior of the study parameter to elaborate the effect of treatments on HPLC quantification. To evaluate the differences among the mean LSD test was applied. Values in same column within each parameter with different letters were significantly different from each other (p ≤ 0. 05)
T1 = Coriander Seed (CS) extract
T2 = Black Cumin Seed (BCS) extract
T3 = Fenugreek Seed (FS) extract
T4 = CS + BCS extract
T5 = CS + FS extract
T6 = BCS + FS extract
T7 = CS + BCS + FS extract