Figure 7.
(a) NTA analysis of the SEC-isolated EVs. Mean particle size: 208 ± 3 nm. (b) WB analysis of SEC-isolated EVs from human serum. The presence of the transmembrane proteins CD9 and CD63 and the luminal proteins ALIX and TSG101 is checked and positivity confirmed for CD63 and TSG101; (c) TEM imaging after negative staining showed results in line with analogous analysis reported elsewhere [36,39]; (d) NTA analysis of SEC-isolated EVs after 6 h incubation at 37°C in presence of trypsin mean particle size: 247 ± 3 nm; (e) TEM imaging after trypsin treatment. (f) Particle density of EV from human serum incubated at the concentration of 1 × 109 particles/mL. EV capturing by BP peptides is not affected by trypsin treatment. (g) EV binding on CD81/CD63/CD9 antibody chip is abolished by surface protein digestion using trypsin.