Table 3.

PCR conditions and identification methods for periodontal pathogens in atherothrombotic samples

Authors (year)	PCR protocol	Cycle (n)	Data analysis
Haraszthy et al. (2000) [11]	Conventional qualitative	30	Hybridization
Okuda et al. (2001) [32]		32 or 36	Clonage and sequencing
Taylor-Robinson et al. (2002) [33]		36	Unknown
Ishihara et al. (2004) [19]		36	Sequencing
Kurihara et al. (2004) [26]		36	Agarose gel
Marques da Silva et al. (2005) [27]		32	Agarose gel
Padilla et al. (2006) [8]		30	Agarose gel
Pucar et al. (2007) [20]		35	Polyacrylamide gel
Elkaïm et al. (2008) [31]		30	Hybridization
Nakano et al. (2009) [28]		36	Unknown
Mahendra et al. (2009) [22]		36	Agarose gel and sequencing
Aquino et al. (2011) [18]		36	Agarose gel
Toyofuku et al. (2011) [9]		36	Gel
Fernandes et al. (2014) [16]		40	Unknown
Rath et al. (2014) [23]		40	Agarose gel
Mahalakshmi et al. (2017) [24]		35 or 36	Gel and sequencing
Atarbashi-Moghadam et al. (2018) [25]		35	Agarose gel and sequencing
Fiehn et al. (2005) [13]	Nested and conventional	30 or 35	Agarose gel and sequencing
Aimetti et al. (2007) [14]	Nested	32	Agarose gel
Figuero et al. (2011) [15]	Nested	35	Agarose gel and sequencing
Figuero et al. (2014) [10]	Nested and quantitative	40	Unknown
Cairo et al. (2004) [12]	Quantitative	35	Agarose gel
Kozarov (2005) [2]		40	Melting curve
Kozarov et al. (2006) [17]		40	Melting curve
Gaetti-Jardim et al. (2009) [21]		40 or 45	Melting curve
Delbosc et al. (2011) [29]		50	Agarose gel
Pyysalo et al. (2013) [30]		60	Melting curve
Rangé et al. (2014) [4]		50	Gel and sequencing