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. 2020 Apr 17;9(1):1747206. doi: 10.1080/20013078.2020.1747206

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Characterization of the IEV protein cargo for EV markers. (a and b) Commonly EV-associated proteins Hsp70, TSG101, CD9 and CD81 along with GM130 and α-tubulin were analysed by western blotting from the pooled fractions 1–6 of cEVs and IEVs or cell lysate (CL) of PC-3 (a) and A549 (b) cells. Also fractions 7–10 from IEV samples were analysed. Representative blots are shown with three independent samples of IEVs/cEVs and two independent samples of CL and fractions 7–10. While other protein markers seemed to be depleted from the IEVs and cEVs, membrane proteins CD9 and CD81 were enriched in those samples. (c and d) the samples were also analysed by stain-free SDS-PAGE, showing a distinct pattern of proteins in both pools of fractions 1–6 and 7–10 when compared to CL.