Figure 5.

Excitatory neuron-specific expression and deposition of mutant tau in rTg4510 mouse brains. A, Specific expression and broad range distribution of human tau harboring P301L mutation in rT4510 brains at 2 months of age were immunohistochemically analyzed with HT7 antibody. B, Age-dependent distribution and phosphorylation of mutant tau in hippocampus CA1 and somatosensory cortex were assessed by HT7 and AT8 antibodies at age 2 and 6 months in rTg4510 mouse brains. C, Expression of human tau in GABAergic interneurons in somatosensory cortex at 2 months of age was histochemically assessed by coimmunostaining with anti-GABA and HT7 antibodies. GABAergic interneurons demonstrate no overlap with HT7 immunoreactivity. D, % of NeuN⁺ and GABA⁺ neurons colabeled with HT7 immunoreactivity at 2-3 months of age. Data from 4 mice/genotype are demonstrated as mean ± SD. **p < 0.01 (Student's t test). E, % of NeuN⁺ neurons colabeled with AT8 immunoreactivity at age 2-3 months and 6-8 months in rTg4510 brains. Data from 4 mice/genotype are demonstrated as mean ± SD. *p < 0.05 (Student's t test).