Figure 5.

Deletion of CPEB3 prevents the stress-induced reduction in GluA1 expression in Bergmann glial cells. A, Confocal images of GFAP-IR and CPEB3-IR indicate that CPEB3 is expressed in Bergmann glial cell processes (top right corner) as well as in granule cells and molecular layer interneurons. CPEB3-IR was absent in CPEB3 knock-out mice. B, Epifluorescence images, and the corresponding quantification of GluA1-IR in wild-type and CPEB3 KO mice, indicates that there is no difference between genotypes (WT, N = 3; KO, N = 3). C, Epifluorescence images and GluA1-IR in naive KO and stressed KO mice. Deletion of CPEB3 did not alter GluA1-IR in the molecular layer (naive KO, N = 4; stress KO, N = 4). Stress reduced GluA1-IR by 16 ± 4% in wild-type mice (naive WT, N = 3; stress WT, N = 3). ML, Molecular layer; GL, granule cell layer; PL, Purkinje cell layer. Scale bars: A, 50 µm; B, C, 100 µm. *p < 0.02 (unpaired t test).