Figure 8.

Adenylyl cyclase 5 mediates the stress-induced decrease in GluA1 expression. A, Double staining for GFAP and AC5 indicates that AC5 is expressed in the processes of Bergmann glial cells. AC5-IR was reduced in AC5 knock-out mice. B, Epifluorescence images and the corresponding quantification of GluA1-IR in naive wild-type animals and after stress (BG processes: naive, N = 4; stress, N = 4). C, In contrast, AC5 knock-out mice no longer showed any decrease in GluA1-IR after stress (naive KO, N = 3; stress KO, N = 3). ML, Molecular layer; GL, granule cell layer; PL, Purkinje cell layer. Scale bars: A, 50 µm; B, C, 100 µm. *p < 0.04 (unpaired t test).