Fig. 3.

Knockdown of Runx1t1 decreased neuronal differentiation of hippocampal NSCs. The gene and protein expression of Runx1t1 in the RNAi group was significantly lower than the mock-infected and NC groups (a, b). Compared to the mock and NC groups, less Tuj1-positive cells were detected after treatment with LV3-Runx1t1-RNAi, and the length of processes of Tuj1-positive cells became shorter, lesser, and even less evident (c–k). Scale bar = 50 μm. The numbers of Tuj1-positive cells showed a significant difference between the mock-infected and LV3-NC groups with an LV3-Runx1t1-RNAi group (l). P < 0.05. NC, negative control; RNAi, RNA interference