Fig. 2.

Decreased airway inflammatory response in MyD88 KO mice following repetitive exposure to ODE for 3 weeks. Wild-type (WT) and MyD88 knock-out (KO) mice were treated i.n. with saline or ODE daily for 3 weeks, whereupon bronchoalveolar lavage fluid (BALF) was collected five hours following final exposure. Panel a, Total cells, neutrophils, macrophages, and lymphocytes were increased in ODE-treated WT mice, and this response was significantly reduced in MyD88 KO animals. Panel b, ODE-induced TNF-α, IL-6, and murine neutrophil chemoattractants (CXCL1 and CXCL2) were reduced or abrogated in MyD88 KO mice. Scatter plots show mean with SEM; N = 10 mice/group (N = 6 male, N = 4 female) from 3 independent experiments