Fig. 6.

Repetitive ODE exposure increases expression of several tight junction proteins known to be upregulated in inflamed/injured lung in WT mice but not in MyD88 KO mice. WT and MyD88 KO mice were treated i.n. daily for 3 weeks with saline or ODE. Panel a, Expression of tight junction mRNA was measured by real-time quantitative PCR and are reported as fold-changes normalized to control. Panel b, Quantification of tight junction protein expression in ODE-treated mice compared to control mice as determined by the immunoblot presented in Panel c. Scatter plots demonstrate mean with standard error bars of N = 3 animals per group with 2 replicates per sample