. Author manuscript; available in PMC: 2020 Sep 20.

Published in final edited form as: ACS Chem Biol. 2019 Oct 22;14(12):2701–2712. doi: 10.1021/acschembio.9b00634

Table 2.

Performance of sPDz probes of the P1 and P2 sets^a

Probe	P1 set
Probe	P1^6:6	P1^3:9	P1^9:3	P1^6:6-teg	P1^3:9-teg	P1^9:3-teg
S/B	4.26±0.05	4.5±0.1	5.8±0.1	5.42±0.04	4.0±0.1	10.47±0.06
SF^b	98	100	99	90	51	98
Probe	P1 set with a stem-loop
Probe	sl-P1^6:6	sl-P1^3:9	sl-P1^9:3	sl-P1^6:6-teg	sl-P1^3:9-teg	sl-P1^9:3-teg
S/B	1.82±0.03	4.63±0.05	1.31±0.03	3.84±0.06	6.78±0.08	3.32±0.04
SF^b	100	99	69	97	91	95
Probe	P2 set
Probe	P2^6:6	P2^3:9	P2^9:3	P2^6:6-teg	P2^3:9-teg	P2^9:3-teg
S/B	1.22±0.03	0.9±0.1	1.21±0.02	1.31±0.08	ND	1.92±0.05
SF^b	65	66	100	30	ND	66
Probe	P2 set with a stem-loop
Probe	sl-P2^6:6	sl-P2^3:9	sl-P2^9:3	sl-P2^6:6-teg	sl-P2^3:9-teg	sl-P2^9:3-teg
S/B	1.49±0.04	0.8±0.3	1.28±0.07	6.95±0.08	1.1±0.4	3.22±0.08
SF^b	24	0	16	73	0	70

The values for S/B and/or SF indicating poor performance of the probe are in grey. Acceptable probes are bold. “ND” stands for “not determined”;

In case of a mathematical value for SF>100%, the SF was made equal to 100%. When the signal triggered by the mismatched target was higher when the signal in the presence of the fully complementary target, the SF was 0.