Table 3.
Performance of sPDz probes of the P3 seta
| Probe | S/B | SFb | |||||
|---|---|---|---|---|---|---|---|
| SNS at nt2058 | SNS at nt2059 | ||||||
| G | T | C | G | T | C | ||
| P36:6 | 5.8±0.3 | 26 | 39 | 87 | 50 | 24 | 86 |
| P33:9 | 5.2±0.2 | 0 | 0 | 11 | 2 | 3 | 10 |
| P39:3 | 7.5±0.2 | 72 | 80 | 100 | 87 | 71 | 100 |
| P36:6-teg | 5.3±0.1 | 0 | ND | ND | 0 | ND | ND |
| P33:9-teg | 11.81±0.05 | 3 | ND | ND | 0 | ND | ND |
| P39:3-teg | 10.3±0.2 | 22 | ND | ND | 24 | ND | ND |
| sl-P36:6 | 12.1±0.2 | 75 | 95 | 100 | 90 | 96 | 100 |
| sl-P33:9 | 11.48±0.08 | 10 | ND | ND | 20 | ND | ND |
| sl-P39:3 | 9.5±0.3 | 100 | 98 | 99 | 100 | 97 | 100 |
| sl-P36:6-teg | 8.4±0.2 | 89 | ND | ND | 95 | ND | ND |
| sl-P33:9-teg | 16.94±0.04 | 43 | ND | ND | 65 | ND | ND |
| sl-P39:3-teg | 11.6±0.2 | 97 | ND | ND | 100 | ND | ND |
a
The values for S/B and/or SF indicating poor performance of the probe are in grey. Acceptable probes are bold. “ND” stands for “not determined”;
b
In case of a mathematical value for SF>100%, the SF was made equal to 100%. When the signal triggered by the mismatched target was higher when the signal in the presence of the fully complementary target, the SF was 0.