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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: J Immunotoxicol. 2018 Dec;15(1):63–72. doi: 10.1080/1547691X.2017.1420712

Figure 2.

Figure 2.

Protocol for TCE induction of sensitization in mice. After 7 days of adaptive feeding, TCE was applied to the abdomen by intradermal injection. On Days 4, 7, and 10, TCE (100 μl of 30% TCE [TCE:olive oil:acetone, 3:2:5 {v/v/v}] solution) was applied to the dorsal skin. Animals were then re-challenged with TCE on Days 17 and 19. NF-κB inhibitor PDTC was applied to the PDTC pre-treatment group of mice 24 hr before each challenge.