Photomicrograph of testes sections after exposure of rats to Ag-NPs showing; (A) several normal seminiferous tubules with normal spermatogonia cell, normal Sertoli cells, normal germ cell layer with normal maturation stages; (B) interstitial spaces show an area of interstitial congestion; (C) the lumen devoid of spermatozoa with interstitial congestion; (D) normal seminiferous tubules with normal spermatogonia cell, normal Sertoli cells, and normal germ cell layer; (E) maturation arrest of tubules and mild interstitial congestion; (F) seminiferous tubules with atrophy exhibiting thick double cell layers indicative of cessation of spermatogenesis. Magnification 400×. A: Control, B: 10 mg/kg bw, C: 50 mg/kg bw, D: Control, E: 10 mg/kg bw, F: 50 mg/kg bw. A–C (7 days exposure of Ag-NP), D–F (28 days exposure of Ag-NP).