Table 3.
Effects of sub-dermal administration of Ag-NP on hematological parameters in rats.
| 7 Days | 28 Days | |||||
|---|---|---|---|---|---|---|
| Control | 10 mg/kg | 50 mg/kg | Control | 10 mg/kg | 50 mg/kg | |
| WBC (×103/mm3) | 11.77 ± 3.39 c | 10.68 ± 7.51 b | 6.23 ± 0.59 a | 6.18 ± 2.38 b | 5.99 ± 1.75 ab | 5.86 ± 2.81 a |
| NEU (%) | 10.54 ± 3.31 c | 5.67 ± 0.29 b | 3.41 ± 0.18 a | 3.14 ± 0.97 b | 3.21 ± 0.59 b | 2.167 ± 0.40 a |
| MID (%) | 0.96 ± 0.46 c | 0.87 ± 0.16 b | 0.63 ± 0.34 a | 0.61 ± 0.12 b | 0.57 ± 0.09 a | 0.67 ± 0.37 a |
| GRA | 0.27 ± 0.11 b | 0.23 ± 0.04 b | 0.13 ± 0.09 a | 0.07 ± 0.03 a | 0.21 ± 0.13 b | 0.20 ± 0.16 b |
| LYM (%) | 89.07 ± 4.30 c | 77.90 ± 1.21 b | 47.00 ± 2.6 a | 46.00 ± 3.6 b | 76.23 ± 1.28 b | 39.47 ± 4.19 c |
| MID (%) | 14.13 ± 0.40 b | 11.43 ± 2.22 a | 10.97 ± 2.6 a | 12.17 ± 3.1 b | 9.77 ± 1.47 a | 8.23 ± 4.70 a |
| GRA (%) | 3.43 ± 0.50 a | 3.17 ± 0.23 a | 5.60 ± 7.10 a | 3.43 ± 1.40 b | 1.91 ± 0.31 a | 3.40 ± 0.46 b |
| RBC (1012L) | 6.75 ± 0.13 a | 6.56 ± 1.19 a | 6.29 ± 2.85 a | 5.56 ± 1.47 a | 6.15 ± 0.73 b | 7.06 ± 1.79 c |
| HB (g/dl) | 161.33 ± 8.1 b | 156.0 ± 13.9 b | 134.00 ± 32 a | 128.3 ± 12.22 a | 138.33 ± 7.3 b | 153.67 ± 10.45 c |
| MCV (f/l) | 74.27 ± 1.42 b | 77.67 ± 1.53 b | 52.73 ± 0.51 a | 54.30 ± 1.66 a | 86.00 ± 1.00 b | 55.53 ± 1.76 a |
| MCH (pg) | 30.0 ± 0.50 b | 29.50 ± 0.78 b | 15.60 ± 3.1 a | 25.67 ± 4.38 a,b | 28.17 ± 0.73 b | 22.57 ± 5.16 a |
| RDW-SD | 32.30 ± 1.91 b | 30.03 ± 4.30 b | 25.40 ± 2.2 a | 26.27 ± 2.40 a | 28.43 ± 3.99 a | 29.97 ± 2.30 a |
| RDW-CV | 17.17 ± 0.25 | 15.70 ± 0.40 a | 15.30 ± 0.49 a | 13.60 ± 2.16 a | 14.73 ± 0.31 a | 14.17 ± 2.75 a |
| PLT (103 µL) | 286.00 ± 9.5 a | 305.0 ± 5.00 b | 261.33 ± 10 a | 272.33 ± 5.5 a | 334.67 ± 12 b | 296.67 ± 4 a |
| MPV (g/dl) | 7.49 ± 0.08 b | 7.30 ± 0.10 b | 6.83 ± 0.15 a | 6.67 ± 0.61 a | 7.27 ± 0.15 b | 6.63 ± 0.32 a |
| PCW | 9.90 ± 0.10 a | 10.20 ± 0.12 a | 14.0 ± 2.21 b | 12.57 ± 0.40 b | 10.50 ± 0.61 a | 12.93 ± 1.10 b |
| HCT | 0.21 ± 0.01 a | 0.22 ± 0.01 a | 0.27 ± 0.17 a | 0.36 ± 0.11 a | 0.34 ± 0.03 a | 0.35 ± 0.07 a |
Data expressed as mean ± standard deviation of triplicate determination. Data followed by different superscript alphabet along the same column are significantly different (p < 0.05). The high significant levels of the parameters were in the order of a < b < c. The high significant levels of the parameters were in the order of a < b < c. Data with superscript alphabet “a” are significantly lower than data with superscript alphabet “b” while data with superscript “b” are lower than data with superscript alphabet “c” at p < 0.05. WBC: white blood cell count; RBC: red blood cell count; Hb: hemoglobin concentration; HCT: hematocrit; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; MCHC: mean corpuscular hemoglobin concentration; RDW: red cell distribution width; PLT: platelet count; MPV: mean platelet volume; LYM%: percent of lymphocytes; NEU: neutrophil; MID: mixed.