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. 2020 Mar 3;25(5):1134. doi: 10.3390/molecules25051134

Table 5.

Essential oils and their components and their use for the development of active food packaging.

Essential oil Component Encapsulation Strategy Packaging Material Food Product Antimicrobial Effectiveness in vivo Ref.
Cinnamon NA Polyvinyl alcohol electrospun fibres Strawberries When compared to control films, EO films stopped fungal rotting for up to 6 days of storage at 21 °C [89]
Oregano Nanoemulsion Mandarin fibre edible coating Low-fat cut cheese Decreased Staphylococcus microbial population by 1.4 and 1.5 log CFU/g in coated cheese pieces containing 2.0% or 2.5% w/w of EO, respectively, during 15 days of refrigerated storage [90]
Lemongrass NA Zein edible coating Cold-smoked sunshine bass fillets LG-treated samples reduced L. monocytogenes counts by 2.5 log in polyvinyl chlorine and 1.7 log in vacuum-packaged samples, respectively [91]
Ginger NA Soy protein/zein electrospun fibres Fresh Minas cheese Significant reductions of L. monocytogenes were observed on the 3rd and 9th day of storage
At day 9, L. monocytogenes counts decreased from 4.39 log CFU/g to 3.62 log CFU/g for the stored cheeses in the package containing EO-fibres when compared to the cheese stored in the fiberless package at 4 °C
[92]
Thymol
Carvacarol
Montmorillonite Themoplastic starch films Strawberries In vivo additive/synergistic antimicrobial effect over Botrytis cinerea-inoculated strawberries was observed when carvacrol+thymol were both included in the films with respect to the films containing only carvacrol
A drastic reduction of 2.4-fold on EO inhibitory concentration against Botrytis cinerea in strawberries stored at room temperature for 5 days: IC50 values dropped from 14.16 g/kg film (only carvacrol) to 5.90 g/kg film (carvacrol: thymol 50:50) in indirect contact with the films
[93]
Paulownia tomentosa Chitosan nanoparticles Chitosan edible coating Pork chop slices EO-chitosan coatings decreased microbial growth (total viable counts, Pseudomonads and lactic acid bacteria) on pork chops compared to the control during 16 days of refrigerated storage
Microbial shelf-life extension from 6 to 9 days
[94]
Thyme
Cinnamon
Lemongrass
NA Chitosan film Peanut kernels Peanut kernels packed in chitosan films incorporated with 4% cinnamon EO showed complete inhibition of Aspergillus flavus and Penicillium citrinum growth at 4 and 28 °C after 24 days of storage compared with all other treatments
Thyme and lemongrass EOs were less effective in reducing fungal growth at all concentrations and conditions tested
[95]
Chamomile
Ginger
NA Whey protein isolate edible coating Rainbow trout fillets Significant reduction in total viable counts and psychrotrophs was observed in trout fillets during 15 days of refrigerated storage when coated with ginger and camomile alone or in combination
The best results were obtained when both oils were used in combination
These films did not show a significant reduction in lactic acid bacteria counts and Pseudomonads
[96]
Oregano
Tea tree
Peppermint
Nanoemulsion Cellulose nanocrystals (CNCs) reinforced chitosan Rice Of the 3 combinations tested (thyme:oregano, thyme:tea tree and thyme:peppermint), thyme:oregano nanoemulsions were the most effective against A. niger, A. flavus, A. parasiticus and P. chrysogenum
Thyme:oregano films caused a significant reduction in all moulds growth during the 8 weeks of storage at room temperature
This antifungal activity was improved when active films were used in combination with irradiation treatment
[97]
Geraniol
α-Terpilenol
NA Ethylene–vinyl alcohol copolymer (EVOH) Fish slices On day 8 of 10 days of refrigerated storage, the total viable counts cut down 1.98 ± 0.02 log units for fish samples packaged in geraniol/EVOH films
Active films containing 6% (w/w) of geraniol and terpineol effectively extended shelf life by 4–5 days under cold-storage conditions compared with the control group
[98]
Eugenol
Carvacrol
Thymol
NA Zein edible coating Melons The coating of melons with zein-2% eugenol mixtures caused a marked and similar decrease in both L. innocua and E. coli counts on melon surface during storage at 4 °C for 10 days [99]
Cumin NA PET films coated with chitosan and alginate Chicken meat No significant growth reduction was obtained for total viable counts and psychrotrophs in active film chicken samples during refrigerated storage during 6 days [100]
Thyme NA Silk fibroin electrospun fibres Poultry (chicken and duck) meat Active films caused a 2-fold reduction on Salmonella Typhimurium on chicken and duck meat stored at 25 and 4 °C
Films antimicrobial activity was enhanced when combined with cold plasma
[101]
EO mix (carvacrol:oregano:cinnamon 70:10:20) Cyclodextrin
Halloysite tubes
Cardboard Tomatoes Decay incidence of tomatoes within cyclodextrin−EOs boxes was reduced from 9−15% to 2% after a storage period of 6 days/8 °C+12 days/25 °C [102]
Carvacrol Halloysite tubes Chitosan-coated polyethylene Chicken meat Active films caused a 1.5 log reduction on total viable counts on chicken meat surface following 24h of incubation at 4 °C [103]