Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 26;25(5):1054. doi: 10.3390/molecules25051054

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Occurrence of autophagoproteasome in U87MG cells after treatment with 1 μM SG-1, SG-2 or T1AM. As shown in representative micrographs (a–c), when LC3 and P20S immunogold staining was carried out concomitantly the occurrence of both P20S particles and LC3 particles within the very same autophagy vacuoles was documented in U87-MG cells exposed to 1 μM SG-1, SG-2 or T1AM. In these pictures P20S staining is represented by 20nm immunogold particles evidenced by arrowheads, while LC3 staining is represented by smaller (10 nm) immunogold particles evidenced by full thin arrows. After treatment with 1 μM SG-1, SG-2 and T1AM the mean number of autophagoproteasomes occurring in the cell was counted, and as shown in graphs (d–f), a time-dependent increase of P20S+LC3 positive vacuoles was documented. Values represent the mean ± SEM of 30 cells for each group (n = 30) Comparisons between groups were made by using one-way ANOVA followed by a Dunnett’s post-test. * P ≤ 0.05, ** P ≤ 0.01compared with baseline condition. Scale bars (a–c) = 50 nm.

Occurrence of autophagoproteasome in U87MG cells after treatment with 1 μM SG-1, SG-2 or T1AM. As shown in representative micrographs (a–c), when LC3 and P20S immunogold staining was carried out concomitantly the occurrence of both P20S particles and LC3 particles within the very same autophagy vacuoles was documented in U87-MG cells exposed to 1 μM SG-1, SG-2 or T1AM. In these pictures P20S staining is represented by 20nm immunogold particles evidenced by arrowheads, while LC3 staining is represented by smaller (10 nm) immunogold particles evidenced by full thin arrows. After treatment with 1 μM SG-1, SG-2 and T1AM the mean number of autophagoproteasomes occurring in the cell was counted, and as shown in graphs (d–f), a time-dependent increase of P20S+LC3 positive vacuoles was documented. Values represent the mean ± SEM of 30 cells for each group (n = 30) Comparisons between groups were made by using one-way ANOVA followed by a Dunnett’s post-test. * P ≤ 0.05, ** P ≤ 0.01compared with baseline condition. Scale bars (a–c) = 50 nm.