Figure 4.

In-vivo evaluation of the anti-anaphylactoid effect of Genistein in local anaphylactoid mice model. Representative images of Evans blue extravasation and cutaneous flare reactions in mice (a). Mice were treated with intravenous injection of 10 mg/kg and 20 mg/kg Genistein combined with 50 μL of Evans blue. After 30 min, sub plantar injection of 30 μg/mL compound 48/80 was injected in the right paw and saline in the left paw. Quantification of % increase in paw thickness after 15 min of compound 48/80 injections (b). Quantification of Evans blue leakage into the paw after 15 min of compound 48/80 injection (c). The data are presented as mean ± SEM (n = 6). Ordinary one-way ANOVA followed by Tukey’s multiple comparisons test was used to determine significance in statistical comparisons, and statistical significance was accepted at p < 0.05 (** p < 0.01 when compared with compound 48/80 control; ## p < 0.01 when compared to saline).