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. 2020 Mar 2;25(5):1102. doi: 10.3390/molecules25051102

Figure 8.

Figure 8

Inhibitory effects of a GPR55 antagonist, a calcium chelator, an LPA1/3 antagonist, and GPR55 silencing via siRNA on gintonin-induced migration and effect of siRNA treatment on GPR55 expression. Cells were incubated with gintonin (1 μg/mL) or LPI (1 μM) in serum-free RPMI1640 medium in the presence or absence of the GPR55 antagonist CID16020046 (10 μM) (a,b), the calcium chelator BAPTA (5 μM) (b), or the LPA1/3 antagonist Ki16425 (10 μM) (b,c) for 5 h. The migration of cells towards the gintonin-containing chamber through the polycarbonate membrane of modified Boyden chambers was quantitated, as described in Materials and Methods (a–c). Data represent means ± SD (n = 4); *** p < 0.001, vs. control (Con). # p < 0.05, ## p < 0.01, ### p < 0.001, vs. GT alone. GT, gintonin; LPI, lysophosphatidylinositol; CID, CID16020046 (a–c). (d) PC-3 cells were transfected with either scrambled siRNA (SC) or GPR55 siRNA (iGPR40) and incubated for 2 days. Immunoblot analysis of GPR55 levels in PC-3 cells that had been transfected with either scrambled siRNA (SC) or GPR55 siRNA. Data represent means ± SD (n = 4); *** p < 0.001, vs. sc siRNA.