Table 4.
Analytical steps involved in the automated multidimensional extraction/determination of the analytes.
| Event | Time (min) | Solvent Composition (Extraction Column) | Solvent Composition (Analytical Column) |
|---|---|---|---|
| Loading | 0.00–1.00 | H2O + 0.2% FA | H2O (A)/ACN (B) * (30%:70%) |
| Eluting | 1.00–3.00 | H2O (A)/ACN (B) * (30%:70% → 35%:65%) | H2O (A)/ACN (B) * (30%:70% → 35%:65%) |
| 3.00–6.00 | H2O (A)/ACN (B) * (35%:65% → 40:60%) | H2O (A)/ACN (B) * (35%:65% → 40:60%) | |
| 5.00–6.00 | H2O (A)/ACN (B) * (40%:60%) | H2O (A)/ACN (B) * (40%:60%) | |
| Cleaning | 6.00–7.00 | H2O (A)/ACN (B) * (40%:60% → 50%:50%) | H2O (A)/ACN (B) * (40%:60% → 50%:50%) |
| 7.00–7.66 | H2O (A)/ACN (B) * (50%:50% → 10%:90%) | H2O (A)/ACN (B) * (50%:50% → 10%:90%) | |
| 7.66–8.60 | H2O + 0.2% FA | H2O (A)/ACN (B) * (10%:90%) | |
| Conditioning | 8.60–11.50 | H2O + 0.2% FA | H2O (A)/ACN (B) * (30%:70%) |
* Both mobile phases acidified with 0.2% formic acid.