Figure 4.
Expression of MAPK signaling factors and inflammatory cytokines with antagonist and GEF on heat stress (a) Analysis of antagonist (Ki16425) on p-p38 and p-ERK expression by Western blot. Quantification of p-p38 per total p38 was represented by a bar graph and quantification of p-ERK per total ERK was represented by a bar graph. (b) Expression of inflammatory cytokines treated with antagonist and GEF at heat stress. Analysis of the effect of antagonist on IL-18, IL-1β, IL-6 and COX-2 expression by Western Blot. Quantification of IL-18, IL-1β, IL-6 and COX-2 per GAPDH was represented by a bar graph, respectively. Values labeled with different letters are significantly different (p < 0.05).
