Figure 3.

MiR-155 absence attenuated neutrophil activation and accumulation. Bronchoalveolar lavage fluid (BALF) and lung tissue were harvested at 12 h after 15 min of smoke exposure. (A) Cell counts of BALF neutrophils was determined. (B) Diff-Quick-stained cytospins of bronchoalveolar lavage neutrophils after smoke inhalation, original magnification 400×. (C–E) The myeloperoxidase (MPO) level in lung tissue and macrophage inflammatory protein 2 (MIP-2) and keratinocyte chemoattractant (KC) proteins in BALF were measured by ELISA. n = 4 mice per group. *p < 0.05 vs. WT mice treated with air. #p < 0.05 vs. WT mice treated with smoke.