(A–C) WT and TLR2–/– mice were injected IV, with NaCl or NaIO3 (50 mg/kg) and eyes enucleated 72 h later.
(A) TUNEL (red) immunoreactivity (scale bars represent 20 µm) and (B) TUNEL quantification.
(C) Number of photoreceptor cell rows in the ONL. Data shown are mean ± SEM; p value determined by nonparametric t test.
(D and E) IF of C3 in (D) WT versus (E) TLR2–/– mice.
(F and G) Expression of C3 cleavage fragments in RPE/choroid tissue from WT and TLR2–/– mice injected i.v., with NaCl or NaIO3 (F) representative images and (G) quantification of iC3b.
*p < 0.05,**p < 0.01, ***p < 0.001.