FIG 3.

Maximum likelihood (ML) phylogenetic tree of IncA plasmids. The IncA phylogenetic tree was generated by comparing 20 complete plasmid sequences, including 18 sequences downloaded from GenBank and the pGA_VIM and pFDL_VIM sequences, which were determined in this study (Table 2). Parsimony SNP analysis was performed by kSNP3 (Galaxy version 3.1) software at the ARIES public Galaxy server (https://w3.iss.it/site/aries/). The phylogenetic tree was visualized using FigTree v. 1.4.3 (http://tree.bio.ed.ac.uk/software/figtree/). The group of the VIM-1-IncA plasmid in the tree is indicated with a dashed vertical bar.