FIG 4.

Loss of protection in CoPP-treated mice is associated with increased IL-6. Cytokine analysis of samples collected at 72 hpi (shown in Fig. 3). Filtered lung homogenates (A to E) and serum (F to G) were analyzed for the following proteins by ELISA or multiplex assay: HO-1 (A), IL-6 (B), IFN-γ (C), TNF-α (D), IL-10 (E), and serum IL-6 (F). The right panel indicates the percentage of mice with high levels of serum IL-6 (>1,000 pg/ml). (G) IFN-γ, TNF-α, and IL-10 from mice with high serum IL-6. Bars indicate the median. The data shown were collected in two to four independent trials and were analyzed for statistical significance by a Mann-Whitney test comparing treated to untreated (*, P < 0.05).