FIG 3.

Treatment with FHNA decreases CHIKV specific infectivity. (A) VeroE6 cells were pretreated with 10 μM FHNA for 12 h, treated at the time of infection or at 2-h intervals postinfection. The cells were infected with CHIKV at an MOI of 1, the intracellular CHIKV RNA was isolated at 12 hpi, and the extracellular CHIKV RNA was isolated at 16 hpi (B). Genome copy numbers were determined by an internally controlled multiplex TaqMan qRT-PCR with probes specific for the nsP1 (gRNA) and E1 (g + sgRNA) coding regions. The data for panels A and B represent the means ± the SD of two independent experiments performed in duplicate. (C) The CHIKV specific infectivity at each treatment interval was determined by dividing the number of genome copies by the infectious virus yield (PFU/ml). UTR, untreated.