TABLE 4.
Phenotypic resistances and characteristics of all rCHIKV mutants generated by reverse engineering and compared to wt CHIKV
| Recombinant virus | Mean EC50 (μM) ± SD | Fold resistancea |
|---|---|---|
| wt | 0.21 ± 0.07 | 1 |
| nsP1-R171Q | 0.58 ± 0.37 | 3 |
| nsP1-G230R | 0.50 ± 0.33 | 2 |
| nsP1-K299E | 0.50 ± 0.08 | 2 |
| nsP1-L455P | 1.1 ± 1.1 | 5 |
| nsP2-A511S | 1.9 ± 0.6 | 9 |
| nsP2-T675A | 0.80 ± 0.08 | 4 |
| nsP3-G177R | 0.51 ± 0.02 | 2 |
| nsP3-A137Vb | NDc | |
| nsP3-H342Q | 0.94 ± 0.01 | 5 |
| nsP3-*524R | 8.5 ± 2.3 | 40 |
| nsP1-G230R + nsP1-K299E | 18.2 ± 4.2 | 87 |
| nsP1-R171Q + nsP3-A137V | 3.9 ± 1.3 | 18 |
| nsP1-R171Q + nsP2-A511S | 6.9 ± 2.6 | 33 |
| nsP2-T675A + nsP3-H342Qd | 7.6 ± 0.2 | 36 |
| nsP2-A511S + nsP3-H342Q | 0.18 ± 0.19 | 1 |
| nsP1-G230R + nsP1-K299E + nsP3-*524R | 25.6 ± 0.1 | 122 |
| nsP2-T675A + nsP3-H342Q + nsP3-*524R | 10.1 ± 6.8 | 48 |
| nsP1-R171Q + nsP2-A511S + nsP3-H342Q | 10.2 ± 2.6 | 49 |
a
The fold resistance = (EC50 recombinant mutant/EC50 wt). See the text for explanation of boldfacing in this table.
b
Not viable.
c
ND, not determined.
d
The mutation H342Q reverted back to wt.