TABLE 1.
Overview of parameters in the final model with values estimated on all data (including both static and dynamic cefuroxime concentrations)a
| Parameter (unit) | Parameter description | Estimate (RSE [%]) |
|---|---|---|
| kgrowth (h−1) | Bacterial growth rate | 1.45 (5.1) |
| kdeath (h−1) | Bacterial natural death rate | 0.179 (fix) |
| Bmax (CFU/ml) | Bacterial system capacity | 1.42 × 108 (19) |
| TSR (CFU/ml) | Threshold for transition from S state to R state | 7.01 × 107 (14) |
|
Emax (h−1) |
Maximum antibiotic-induced killing rate | 3.26 (3.8) |
| EC50 (mg/liter) | Potency for drug effect (MIC dependent) | |
| MIC = 2 mg/liter | 2.93 (2.7) | |
| MIC = 4 mg/liter | 4.88 (11) | |
| γ | Sigmoidal Hill coefficient | 3.37 (25) |
| kdeg (h−1) | Degradation rate of antibiotic (both systems) | 0.026 (fix) |
| kel (h−1) | Elimination rate (dynamic system) | 0.462 (fix) |
| Mix | Fraction of experiments with typical elimination | 0.574 (24) |
| Offset | Proportional change for atypical elimination | 1.36 (4.7) |
| kG,ETX (EU/CFU) | LPS/endotoxin from bacterial growth (S) | 0.000292 (11) |
| kK,ETX (EU/CFU) | LPS/endotoxin from antibiotic-killed bacteria (S) | 0.00636 (25) |
| kFK,ETX (EU/CFU) | LPS/endotoxin from antibiotic-killed filaments (F) | 0.295 (43) |
| ktr,ETX (h−1) | Transit rate for LPS/endotoxin release (S + F) | 0.131 (27) |
| θMIC,F | Concentration × MIC for filament formation | 5.90 (8.2) |
| ω2 | CFU unexplained residual variance | 0.409 (12) |
| ωrepl2 | CFU replication error (plate spread) variance | 0.0363 (16) |
| ω2 | LPS/endotoxin unexplained residual variance | 0.164 (13) |
a
EU, endotoxin units; F, filament biomass state; R, resting state; RSE, relative standard error; S, susceptible state.