TABLE 2.
Posttreatment evaluations and efficacy of oral treatment with LYC formulations and BZ in mice infected with Trypanosoma cruzi VL-10 in chronic phase of infection
| Experimental group | Dose (mg/kg/day) | % of animals (no. of animals with the indicated outcome or result/total no. tested) |
|||||
|---|---|---|---|---|---|---|---|
| Survival | Negative laboratory tests |
Cured by classic criterion | Negative heart tissue qPCRd | ||||
| HCa | PCRb | ELISAc | |||||
| Free LYC | 12 | 70 (7/10) | 14.3 (1/7) | 57.1 (4/7) | 0 (0/7) | 0 (0/7) | 16.7 (1/6) |
| LYC-PLA-PEG-NC | 8 | 70 (7/10) | 86 (6/7) | 71 (5/7) | 42.8 (3/7) | 42.8 (3/7) | 42.8 (3/7) |
| LYC-PLA-PEG-NC | 12 | 80 (8/10) | 100 (8/8) | 100 (8/8) | 87.5 (7/8) | 87.5 (7/8) | 100 (8/8) |
| BZ | 100 | 80 (8/10) | 100 (8/8) | 75 (6/8) | 0 (0/8) | 0 (0/8) | 0 (0/8) |
| INTe | 60 (6/10) | 100 (6/6) | 50 (3/6) | 0 (0/6) | 0 (0/6) | 0 (0/8) | |
| Blank NC | —f | 60 (6/10) | 100 (7/7) | 71 (5/7) | 0 (0/7) | 0 (0/8) | 0 (0/8) |
a
HC was performed at 30, 60, 90, and 120 d.p.t.
b
PCR was performed at 60 and 90 d.p.t.
c
ELISA was performed at 90 and 180 d.p.t.
d
qPCR was performed at 180 d.p.t.
e
INT, infected and not treated.
f
—, the amounts of excipients used in blank NC were the same as the amounts used in the LYC-loaded NC formulation.