TABLE 1.
Concentration of AMA needed to restore the activity of different β-lactam antibiotics to the level seen with their EUCAST susceptibility breakpoint concentration in MBL-producing E. coli BW25113a
| MBL | [AMA] at the susceptibility breakpoint of the antibiotic (μg/ml)b |
|||||
|---|---|---|---|---|---|---|
| Meropenem | Doripenem | Ertapenem | Imipenem | Cefotaxime | Ampicillin | |
| NDM-1 | 8 | 12 | 24 | 12 | 64 | 64 |
| NDM-4 | 16 | 16 | 64 | 16 | >64 | >64 |
| NDM-5 | 12 | 24 | 64 | 16 | >64 | >64 |
| NDM-6 | 16 | 16 | 64 | 16 | 64 | >64 |
| NDM-7 | 16 | 24 | 64 | 24 | >64 | >64 |
| VIM-1 | 8 | 8 | 12 | 12 | 24 | 64 |
| VIM-2 | 8 | 8 | 8 | 8 | 12 | 16 |
| VIM-7 | 8 | 8 | 8 | 8 | 8 | 16 |
| CAM-1 | 4 | 12 | 8 | 8 | 24 | 64 |
| DIM-1 | 12 | 12 | 16 | 8 | 24 | 64 |
| IND-1 | 8 | 8 | 12 | 12 | 24 | >64 |
| GIM-1 | 12 | 12 | 32 | 12 | >64 | >64 |
| IMP-1 | 16 | 12 | 64 | 24 | >64 | 64 |
| IMP-7 | 24 | 12 | >64 | 16 | >64 | 32 |
| IMP-27 | 32 | 24 | >64 | ≤0.5 | >64 | 12 |
| SPM-1 | 16 | 24 | 64 | 8 | >64 | >64 |
| CphA2 | >64 | 64 | >64 | >64 | ≤0.5 | ≤0.5 |
| L1 | 12 | 12 | 24 | 8 | ≤0.5 | >64 |
| AIM-1 | 64 | 64 | >64 | 24 | >64 | >64 |
a
All 19 MBL genes were cloned into the pGDP2 vector. All bioassays were conducted in duplicate. This table shows the results from replicate 1.
b
The EUCAST susceptibility breakpoint concentrations for meropenem, doripenem, ertapenem, imipenem, cefotaxime, and ampicillin are 2, 1, 0.5, 2, 1 and 8 μg/ml, respectively.