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Establishing experimental conditions for fluorescein assay. (A) Fluorescein fluorescence signal measured for planktonic cultures of PAO1 at increasing OD620 values after 15, 30, and 60 min of incubation with 100-mg/liter fluorescein diacetate (FDA). (B) Correlation between the fluorescence signal measured after 15 min of incubation and CFU counts. Data are means ± SD from 3 independent determinations.
