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Transcriptome sequencing (RNA-seq) identifies differential gene expression changes following 24 h of antibiotic treatment. (A) Schematic of RNA-seq procedure; 6 transwells per condition. (B) Venn diagram and color key of gene expression patterns for high-dose treatment. (C to E) Volcano plots of gene expression changes for high concentrations of indicated antibiotic compared to vehicle. Highlighted points have at least a 2-fold change with an adjusted P value of <0.01.
