FIG 5.
Influence of antibiotic treatment on Stx-mediated renal damage during murine infection with C. rodentium ϕstx2dact. (A) Kidney sections of uninfected mice or infected mice treated with the indicated antibiotic were stained with H&E and scored. Representative kidney sections for each group are shown, with scale bars indicating 25 μm. Pathological changes are indicated as follows: arrow, sloughing of necrotic cells in lumen; arrowhead, loss of lining epithelium; *, tubular dilation. (B) Pathology scores of proximal tubules of the individual animals in each group. (C) Serum BUN (blood urea nitrogen) levels were determined as a marker for kidney damage. Shown are the means (±SEM) for all assessed mice per group. Statistical analysis was performed by Student's t test, corrected using the Holm-Sidak method (*, P < 0.05; **, P < 0.01; ***, P < 0.001), with results for infected/treated mice compared to those for the untreated control group.