TABLE 3.
Mortality in zebrafish embryos exposed to single drugs at 100 μM or their combination with AmB at 0.03 μg/ml
| Compound or mixturea | % Mortality | ||||
|---|---|---|---|---|---|
| 24 h | 48 h | 72 h | 96 h | Total | |
| Single AC | |||||
| AmB | 0 | 0 | 0 | 0 | 0 | 
| Chenodiol | 0 | 100 | 100 | 100 | 100 | 
| Nisoldipine | 100 | 100 | 100 | 100 | 100 | 
| Chlorcyclizine | 0 | 100 | 100 | 100 | 100 | 
| Cloperastine | 0 | 100 | 100 | 100 | 100 | 
| Erythromycin | 0 | 0 | 0 | 0 | 0 | 
| Glimepiride | 0 | 0 | 0 | 0 | 0 | 
| Promazine | 0 | 100 | 100 | 100 | 100 | 
| Riluzole | 100 | 100 | 100 | 100 | 100 | 
| Nortriptyline | 0 | 0 | 0 | 100 | 100 | 
| DMSO 0.4% | 0 | 0 | 0 | 0 | 0 | 
| Cont – | 0 | 0 | 0 | 0 | 0 | 
| Cont + | 18.75 | 43.75 | 100 | 100 | 100 | 
| Mixture | |||||
| Chenodiol + AmB | 0 | 100 | 100 | 100 | 100 | 
| Nisoldipine + AmB | 100 | 100 | 100 | 100 | 100 | 
| Chlorcyclizine + AmB | 100 | 100 | 100 | 100 | 100 | 
| Cloperastine + AmB | 100 | 100 | 100 | 100 | 100 | 
| Erythromycin + AmB | 0 | 0 | 0 | 0 | 0 | 
| Glimepiride + AmB | 0 | 0 | 0 | 0 | 0 | 
| Promazine + AmB | 56.25 | 81.25 | 100 | 100 | 100 | 
| Riluzole + AmB | 100 | 100 | 100 | 100 | 100 | 
| Nortriptyline + AmB | 0 | 0 | 0 | 100 | 100 | 
| DMSO 0.4% | 0 | 0 | 0 | 0 | 0 | 
| Cont – | 0 | 0 | 0 | 0 | 0 | 
| Cont + | 6.25 | 50 | 100 | 100 | 100 | 
Cont –, negative control for death (nontreated embryos); Cont +, positive control for death (embryos treated with 3,4-dichloroaniline; see Material and Methods).