TABLE 3.
Mortality in zebrafish embryos exposed to single drugs at 100 μM or their combination with AmB at 0.03 μg/ml
Compound or mixturea | % Mortality |
||||
---|---|---|---|---|---|
24 h | 48 h | 72 h | 96 h | Total | |
Single AC | |||||
AmB | 0 | 0 | 0 | 0 | 0 |
Chenodiol | 0 | 100 | 100 | 100 | 100 |
Nisoldipine | 100 | 100 | 100 | 100 | 100 |
Chlorcyclizine | 0 | 100 | 100 | 100 | 100 |
Cloperastine | 0 | 100 | 100 | 100 | 100 |
Erythromycin | 0 | 0 | 0 | 0 | 0 |
Glimepiride | 0 | 0 | 0 | 0 | 0 |
Promazine | 0 | 100 | 100 | 100 | 100 |
Riluzole | 100 | 100 | 100 | 100 | 100 |
Nortriptyline | 0 | 0 | 0 | 100 | 100 |
DMSO 0.4% | 0 | 0 | 0 | 0 | 0 |
Cont – | 0 | 0 | 0 | 0 | 0 |
Cont + | 18.75 | 43.75 | 100 | 100 | 100 |
Mixture | |||||
Chenodiol + AmB | 0 | 100 | 100 | 100 | 100 |
Nisoldipine + AmB | 100 | 100 | 100 | 100 | 100 |
Chlorcyclizine + AmB | 100 | 100 | 100 | 100 | 100 |
Cloperastine + AmB | 100 | 100 | 100 | 100 | 100 |
Erythromycin + AmB | 0 | 0 | 0 | 0 | 0 |
Glimepiride + AmB | 0 | 0 | 0 | 0 | 0 |
Promazine + AmB | 56.25 | 81.25 | 100 | 100 | 100 |
Riluzole + AmB | 100 | 100 | 100 | 100 | 100 |
Nortriptyline + AmB | 0 | 0 | 0 | 100 | 100 |
DMSO 0.4% | 0 | 0 | 0 | 0 | 0 |
Cont – | 0 | 0 | 0 | 0 | 0 |
Cont + | 6.25 | 50 | 100 | 100 | 100 |
Cont –, negative control for death (nontreated embryos); Cont +, positive control for death (embryos treated with 3,4-dichloroaniline; see Material and Methods).