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. 2020 Mar 24;64(4):e01936-19. doi: 10.1128/AAC.01936-19

TABLE 3.

Kinetic parameters of nitrocefin and ceftazidime hydrolysis by KPC-2 and mutant proteins

Enzyme	Nitrocefin			Ceftazidime
Enzyme	K_m, μM	k_cat, s⁻¹	k_cat/K_m, s⁻¹ μM⁻¹	K_m, μM	k_cat, s⁻¹	k_cat/K_m, s⁻¹ μM⁻¹
KPC-2	36 ± 5	132 ± 13	3.7 ± 0.2	>2,000	>2.6	0.00087 ± 0.00011^a
KPC-2 D179Y	26 ± 12	0.023 ± 0.002	0.0010 ± 0.0005	<5	0.0088 ± 0.0003	0.0088 ± 0.0009^a
KPC-2 L169P	40 ± 10	0.47 ± 0.04	0.012 ± 0.002	27 ± 6	0.093 ± 0.009	0.0036 ± 0.0009

^a

This k_cat/K_m value was calculated by method described in reference 44.