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. 2020 Mar 7;25(5):1208. doi: 10.3390/molecules25051208

Scheme 2.

Scheme 2

Functionalization of bovine serum albumin (7) with trans-cyclooctene (8) to form albumin- trans-cyclooctene (TCO) (9), followed by the inverse-electron demand Diels-Alder (IEDDA) cycloaddition with SiFA-Tz (6) to form fluoroalbumin (10) (molar ratio 1:1.5 SiFA-Tz:fluoroalbumin).