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. 2020 Mar 9;25(5):1230. doi: 10.3390/molecules25051230

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of melatonin on Sertoli cell proliferation after silencing INHA. (A) Cell activity of chicken Sertoli cells (n = 3). (B) The EdU method was used to measure chicken Sertoli cell proliferation (×10 magnification; n = 3). (C) Statistical analysis of data in (B). The relative mRNA expression levels of (D) proliferating cell nuclear antigen (PCNA) and (E) Cyclin D1 (CCND1; n = 3 for both). (F) The relative protein expression levels of CCND1 and PCNA. Quantitative analyses of the (G) CCND1 and (H) PCNA protein results (n = 3 for both). *** p < 0.001; ** p < 0.01; * p < 0.05.