Figure 8.

Effect of H. cordata and P. ribesioides extracts on apoptosis induction in breast cancer cells. Flow cytometric analysis of apoptosis induction on MCF-7 and MDA-MB-231 cells were treated with various concentrations of HCT and PR ethanolic extracts for 24 h. The annexin V-FITC/PI staining apoptotic cells were analyzed using flow cytometry. Camptothecin (CTP; 5 nM) was used as a positive control. Bar graphs showed the summarized data of three independent experiments from MCF-7 (left) and MDA-MB-231 cells (right), performed in duplicate compared with the untreated control. Each value is presented as mean ± SD from three independent experiments. Data are the means ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control. # p < 0.01 and ## p < 0.001 vs. IC20 of each extract. ns, not significantly different.